CPU 2024

Dados do Trabalho


Título

Probenecid increased the relaxation induced by cyclic guanosine monophosphate-augmenting substances in isolated corpus cavernosum from rats

Resumo

Introduction: Erectile dysfunction (ED) is a common male sexual dysfunction characterized by the consistent or recurrent inability to attain and maintain an erection sufficient for satisfactory sexual performance, significantly impacting quality of life. Drug repurposing is a strategy that aims to redirect substances approved on the clinical settings for new therapeutic indications. Probenecid, a drug indicated for the treatment of gout is known for inhibiting multidrug resistance proteins (MRPs), organic anion transporters (OATs) and pannexin-1 as well as for activating transient receptor potential cation channel (TRPV2). The therapeutic concentration of probenecid is in the range of 0.1-1 mM. Aim: To evaluate the effect of probenecid in the relaxing responses induce by different agents in corpus cavernosum (CC) from rat . Methods: Experiments were conducted on isolated CC from rats. Concentration-response curves (CRCs) were carried out and the potency (pEC50) and maximal response values (Emax) were determined. Approval by the Ethic Committee (CEUA/UNICAMP: 6369-1/2023). Results: Probenecid relaxed the CC of rats with an Emax of, approximately, 50%. This response was significantly reduced in the presence of inhibitors of soluble guanylyl cyclase (ODQ, 100 μM, 26.8 ± 4.3 %, n=4, P

Palavras Chave

probenecid; corpus cavernosum; ERECTILE FUNCTION

Área

Geral

Categoria

Estudos experimentais

Autores

Guilherme Ruiz Leonardi, Gabriela Reolon Passos, Mariana Burille Moretti, João Victor Correa de Barros, Elisa Tonellotti, Edson Antunes, Fabiola Zakia Mónica